RT-qPCR can be performed using various fluorescent ... This value is representative of the starting copy number in the original template; the greater the starting amount, the lower the PCR cycle ...
as described previously (1), using an AgPath Universal Probe One-Step RT-qPCR Kit (Thermo Fisher Scientific, United States). The final reaction volume was 12.5 μL, containing 2.5 μL of RNA template of ...
Premix Ex Taq (Probe qPCR) was purchased from TaKaRa (Dalian ... 1 μL of forward primer (10 μM), 1 μL of reverse primer (10 μM), and 1 μL of template DNA. Thermal cycling involved an initial ...
Norovirus infection has characteristic clinical and epidemiological features that can allow its differentiation from other causes of gastroenteritis. Norovirus is often acquired from a contact ...
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Astrocytes in the brain exhibit regional ...
This important study investigates the function of a critical regulator of human early cardiac development. The convincing examination of GATA6 function is thorough and well-executed. The study will be ...
The authors provide useful data to support the existence of a regulatory pathway starting with SPI1-driven ZFP36L1 expression, that goes on to downregulate HDAC3 expression at the transcript level, ...