Like PCR, qPCR amplifies DNA using a DNA polymerase enzyme.This adds new nucleotides to the 3’-OH end of a short, single-stranded DNA segment, called a primer, which complements the template strand. 2 ...

For RT-qPCR, RNA quality and purity is crucial for success and can affect first-strand cDNA synthesis efficiency. You can use total RNA or poly(A)+ RNA as the template, but both must be intact and ...

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...

Some protocols include a 'preamplification' step to increase the amount of template for a subsequent qPCR experiment. This even allows qPCR to be conducted on single cells, but it also increases ...

They enable shorter probes for qPCR, thereby improving assay sensitivity and targeting. Locked ... Provide the manufacturing team with your precise instructions using this Excel template today.

The ubiquity of quantitative PCR (qPCR) for interrogating gene expression in a variety of research and clinical contexts makes it an indispensable laboratory tool. Among the most critical variables ...

UltraMarathonRT® RT-qPCR Kit offers unmatched data reliability and sensitivity for gene expression ... eliminating the need for high-temperature steps and ensuring the integrity of RNA templates.

The exquisite sensitivity of RT-qPCR demands rigorous experimental design; the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines were published to ...

Identify the principles and advantages of microfluidic singleplex qPCR technology, including its scalability, throughput, and ability to simultaneously detect multiple pathogens. Learn how ...

This new qPCR master mix can detect as low as one copy of DNA per microliter from bacterial, viral, fungal, and mammalian sources, helping to ensure contamination-free drug products and accurate ...